【#Tech24H】Peking University and the Chinese Academy of Sciences have collaboratively developed a novel gene editing system named the “Mortise-Tenon Connection System” (MT), which enables efficient targeted DNA insertion and replacement, potentially opening new avenues for large-fragment gene editing. With a peak precise editing efficiency of 59.47%, scarless characteristics, and stable hereditary performance, the system holds broad application prospects in fields such as functional gene research in rice and rapid introduction of desirable traits. This system fills the gap in autonomous precision breeding foundational technology and will drive the deep transformation of precision editing technology from basic research to agricultural production practices.